Layton is invited by Janice to attend an opera in which she is performing, centred around the legendary lost land of Ambrosia and the secret of eternal life it claims to hold, which it is claimed will be rediscovered when its queen returns. Following this resolution, the pair return to Layton's office, where they listen to 'The Eternal Diva', a record by an old student of the professor's named Janice Quatlane, prompting them to recall one of their earliest adventures together from three years ago.
In the present day, archaeologist and puzzle master Professor Layton and his young appreciate Luke Triton solve the mystery of the theft of Big Ben, deducing the culprit to be Layton's perennial adversary, Don Paolo.
